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Twist Bioscience gene 662 synthesis
Gene 662 Synthesis, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+synthesis/gene+synthesis/10__1016_slash_j__omta__2026__201771-262-0-7
Average 86 stars, based on 1 article reviews
gene 662 synthesis - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Biomarker Discovery:

Article Title: VaxLab: integrated platform for rapid multistrategy mRNA vaccine design
Article Snippet: .. Gene synthesis used for the validation experiment was donated by Twist Bioscience. ..

Sequencing:

Article Title: Genome-wide discovery of cis- regulatory elements in a large genome
Article Snippet: .. These sequences were placed upstream of the coding sequence of mNeonGreen followed by the P10 transcriptional terminator, by gene synthesis (Twist Bioscience, USA), in the context of a Minos vector carrying the PhOpsin1-EGFP transgenesis marker (as described above). ..

Article Title: Cholesterol links blood feeding to mosquito development and reproduction
Article Snippet: .. The coding region of Ae. albopictus DHCR-24 was cloned into pET28a by gene synthesis (Twist Bioscience), and the construct was verified by sequencing. pET28a-DHCR-24 and empty vector control were transformed into BL21 (DE3) bacteria and plated on LB agar containing 50 μg/ml kanamycin. ..

Marker:

Article Title: Genome-wide discovery of cis- regulatory elements in a large genome
Article Snippet: .. These sequences were placed upstream of the coding sequence of mNeonGreen followed by the P10 transcriptional terminator, by gene synthesis (Twist Bioscience, USA), in the context of a Minos vector carrying the PhOpsin1-EGFP transgenesis marker (as described above). ..

Construct:

Article Title: Coincident epithelial signals restrain commensal-specific CD8αβ + T cells in the intestine
Article Snippet: .. H2-Kb SABRs were constructed using gene synthesis (Twist Biosciences) similar to the HLA-A0201 SABRs described previously. .. H2-Kb SABRs were constructed using gene synthesis (Twist Biosciences) similar to the HLA-A0201 SABRs described previously.

Article Title: Cholesterol links blood feeding to mosquito development and reproduction
Article Snippet: .. The coding region of Ae. albopictus DHCR-24 was cloned into pET28a by gene synthesis (Twist Bioscience), and the construct was verified by sequencing. pET28a-DHCR-24 and empty vector control were transformed into BL21 (DE3) bacteria and plated on LB agar containing 50 μg/ml kanamycin. ..

Article Title: Genome-wide discovery of cis- regulatory elements in a large genome
Article Snippet: .. The reporter constructs were generated by gene synthesis (Twist Bioscience, USA) and conventional subcloning. ..

Clone Assay:

Article Title: Cholesterol links blood feeding to mosquito development and reproduction
Article Snippet: .. The coding region of Ae. albopictus DHCR-24 was cloned into pET28a by gene synthesis (Twist Bioscience), and the construct was verified by sequencing. pET28a-DHCR-24 and empty vector control were transformed into BL21 (DE3) bacteria and plated on LB agar containing 50 μg/ml kanamycin. ..

Article Title: A broad-host-range Rhizobium rhizogenes strain enables transient expression across diverse crops and establishes functional assays in faba bean
Article Snippet: .. Level 0 gene inserts were produced by commercial gene synthesis (Twist Bioscience) except for Vf CC-NLR (gene Vfaba.Hedin2.R1.2g009560.1; ), which was cloned by PCR from faba bean cDNA ( ). ..

Plasmid Preparation:

Article Title: Cholesterol links blood feeding to mosquito development and reproduction
Article Snippet: .. The coding region of Ae. albopictus DHCR-24 was cloned into pET28a by gene synthesis (Twist Bioscience), and the construct was verified by sequencing. pET28a-DHCR-24 and empty vector control were transformed into BL21 (DE3) bacteria and plated on LB agar containing 50 μg/ml kanamycin. ..

Article Title: Biochemical characterization of xyloglucan galactosyltransferases MUR3 and XLT2 from Spirodela polyrhiza
Article Snippet: .. The DNA of the catalytic domain of MUR3 (36–544 residues) and XLT2 (86–537 residues) without the transmembrane domain as predicted by DeepTMHMM—1.0 (Hallgren et al., ) were generated via gene synthesis (Twist Biosciences) in the pTwist Gateway ENTR Kozak vector and transferred into the mammalian expression vector pGEn2‐DEST (Moremen et al., ) by GatewayTM recombination. .. SpMUR3 and SpXLT2 were expressed as soluble secreted fusion proteins by transient transfection of suspension culture HEK293‐F cells (FreeStyleTM 293‐F cells, Thermo Fisher Scientific, Waltham, MA, USA).

Control:

Article Title: Cholesterol links blood feeding to mosquito development and reproduction
Article Snippet: .. The coding region of Ae. albopictus DHCR-24 was cloned into pET28a by gene synthesis (Twist Bioscience), and the construct was verified by sequencing. pET28a-DHCR-24 and empty vector control were transformed into BL21 (DE3) bacteria and plated on LB agar containing 50 μg/ml kanamycin. ..

Transformation Assay:

Article Title: Cholesterol links blood feeding to mosquito development and reproduction
Article Snippet: .. The coding region of Ae. albopictus DHCR-24 was cloned into pET28a by gene synthesis (Twist Bioscience), and the construct was verified by sequencing. pET28a-DHCR-24 and empty vector control were transformed into BL21 (DE3) bacteria and plated on LB agar containing 50 μg/ml kanamycin. ..

Bacteria:

Article Title: Cholesterol links blood feeding to mosquito development and reproduction
Article Snippet: .. The coding region of Ae. albopictus DHCR-24 was cloned into pET28a by gene synthesis (Twist Bioscience), and the construct was verified by sequencing. pET28a-DHCR-24 and empty vector control were transformed into BL21 (DE3) bacteria and plated on LB agar containing 50 μg/ml kanamycin. ..

Produced:

Article Title: A broad-host-range Rhizobium rhizogenes strain enables transient expression across diverse crops and establishes functional assays in faba bean
Article Snippet: .. Level 0 gene inserts were produced by commercial gene synthesis (Twist Bioscience) except for Vf CC-NLR (gene Vfaba.Hedin2.R1.2g009560.1; ), which was cloned by PCR from faba bean cDNA ( ). ..

Polymerase Chain Reaction:

Article Title: A broad-host-range Rhizobium rhizogenes strain enables transient expression across diverse crops and establishes functional assays in faba bean
Article Snippet: .. Level 0 gene inserts were produced by commercial gene synthesis (Twist Bioscience) except for Vf CC-NLR (gene Vfaba.Hedin2.R1.2g009560.1; ), which was cloned by PCR from faba bean cDNA ( ). ..

Generated:

Article Title: Genome-wide discovery of cis- regulatory elements in a large genome
Article Snippet: .. The reporter constructs were generated by gene synthesis (Twist Bioscience, USA) and conventional subcloning. ..

Article Title: Biochemical characterization of xyloglucan galactosyltransferases MUR3 and XLT2 from Spirodela polyrhiza
Article Snippet: .. The DNA of the catalytic domain of MUR3 (36–544 residues) and XLT2 (86–537 residues) without the transmembrane domain as predicted by DeepTMHMM—1.0 (Hallgren et al., ) were generated via gene synthesis (Twist Biosciences) in the pTwist Gateway ENTR Kozak vector and transferred into the mammalian expression vector pGEn2‐DEST (Moremen et al., ) by GatewayTM recombination. .. SpMUR3 and SpXLT2 were expressed as soluble secreted fusion proteins by transient transfection of suspension culture HEK293‐F cells (FreeStyleTM 293‐F cells, Thermo Fisher Scientific, Waltham, MA, USA).

Subcloning:

Article Title: Genome-wide discovery of cis- regulatory elements in a large genome
Article Snippet: .. The reporter constructs were generated by gene synthesis (Twist Bioscience, USA) and conventional subcloning. ..

Expressing:

Article Title: Biochemical characterization of xyloglucan galactosyltransferases MUR3 and XLT2 from Spirodela polyrhiza
Article Snippet: .. The DNA of the catalytic domain of MUR3 (36–544 residues) and XLT2 (86–537 residues) without the transmembrane domain as predicted by DeepTMHMM—1.0 (Hallgren et al., ) were generated via gene synthesis (Twist Biosciences) in the pTwist Gateway ENTR Kozak vector and transferred into the mammalian expression vector pGEn2‐DEST (Moremen et al., ) by GatewayTM recombination. .. SpMUR3 and SpXLT2 were expressed as soluble secreted fusion proteins by transient transfection of suspension culture HEK293‐F cells (FreeStyleTM 293‐F cells, Thermo Fisher Scientific, Waltham, MA, USA).



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